THE EFFECT OF ALOIN ADMINISTRATION ON DERMAL THICKNESS IN MALE MICE (MUS MUSCULUS) WITH DYSLIPIDEMIA-INDUCED OBESITY

Suci Alfita Sari; Ade Teti Vani; Ade Yuli Amelia; Nadia Purnama Dewi; Dessy Abdullah; Rosmaini Rosmaini

doi:10.59003/nhj.v6i2.2240

Authors

Suci Alfita Sari Universitas Baiturrahmah
Ade Teti Vani Universitas Baiturrahmah
Ade Yuli Amelia Universitas Baiturrahmah
Nadia Purnama Dewi Universitas Baiturrahmah
Dessy Abdullah Universitas Baiturrahmah
Rosmaini Rosmaini Universitas Baiturrahmah

DOI:

https://doi.org/10.59003/nhj.v6i2.2240

Keywords:

Aloin; Aloe vera; dermal thickness; dyslipidemia; obesity; collagen

Abstract

Introduction: Dyslipidemia and obesity are major metabolic disorders associated with persistent oxidative stress and chronic low-grade inflammation. These pathological conditions impair dermal homeostasis by promoting collagen degradation, suppressing fibroblast activity, and disrupting extracellular matrix remodeling, ultimately resulting in dermal thinning and delayed tissue repair. Aloin, the principal anthraquinone glycoside isolated from Aloe vera, possesses well-documented antioxidant and anti-inflammatory properties that may protect dermal tissue against oxidative injury. This study aimed to investigate the effect of aloin administration on dermal thickness in male Mus musculus with dyslipidemia-induced obesity. Methode: A true experimental study employing a posttest-only control group design was conducted using thirty-two male mice with experimentally induced dyslipidemia and obesity. Animals were randomly allocated into a control group (n = 16) and an aloin-treated group (n = 16). The treatment group received oral aloin at a dose of 750 μg/kg body weight once daily via gastric gavage for 28 consecutive days. Skin specimens were processed for histopathological examination using hematoxylin-eosin staining. Statistical comparisons between groups were performed using the Independent Samples t-test, with statistical significance established at p < 0.05. Result: The mean dermal thickness was 0.485 ± 0.090 mm in the control group and 0.465 ± 0.116 mm in the aloin-treated group. Statistical analysis demonstrated no significant difference in dermal thickness between the two experimental groups (p > 0.05). Conclusion: Oral administration of aloin at 750 μg/kg body weight for 28 days did not significantly increase dermal thickness in male mice with dyslipidemia-induced obesity

Downloads

Download data is not yet available.

References

Addis, R. et al. (2020) ‘Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome’, International Journal of Medical Sciences, 17(8), p. 1030. doi: 10.7150/IJMS.43986.

Aman, A. M. et al. (2021) Panduan Pengelolaan Dislipidemia di Indonesia-2021. PB Perkeni.

Dewi, N. P. et al. (2023) ‘EFFECT OF ALOIN EXTRACT ON THE INCREASE OF FIBROBLAS CELL EXPRESSION ON HEALING OF WOUND WOUNDS OF HORSE WHITE RATS (Rattus norvegicus) BY THE AGING PROCESS’, MSJ : Majority Science Journal, 1(4), pp. 171–179. doi: 10.61942/MSJ.V1I4.92.

Dudás, B. (2023) Human Histology: A Text and Atlas for Physicians and Scientists, Human Histology: A Text and Atlas for Physicians and Scientists. doi: 10.1016/C2021-0-00238-8.

Fu, D. et al. (2021) ‘Aloin decelerates the progression of hepatocellular carcinoma through circ_0011385/miR-149-5p/WT1 axis’, Cell Cycle, 20(23). doi: 10.1080/15384101.2021.1988227.

Ge, F. et al. (2023) ‘Aloin inhibits gastric cancer cell proliferation and migration by suppressing the STAT3/ HMGB1 signaling pathway’, Nan Fang Yi Ke Da Xue Xue Bao / Journal of Southern Medical University, 43(5). doi: 10.12122/j.issn.1673-4254.2023.05.04.

Goddard, T. et al. (2022) ‘Sarcopenic obesity is associated with telomere shortening: findings from the NHANES 1999–2002’, International Journal of Obesity, 46(2). doi: 10.1038/s41366-021-00995-z.

Khalid, K. A. et al. (2022) ‘Aging and Wound Healing of the Skin: A Review of Clinical and Pathophysiological Hallmarks’, Life, 12(12). doi: https://doi.org/10.3390/life12122142.

Kong, X. Y. et al. (2022) ‘Characterization of the metabolism of aloin A/B and aloesin in rats using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry’, Biomedical Chromatography, 36(12). doi: 10.1002/bmc.5483.

Lintzeri, D. A. et al. (2022) ‘Epidermal thickness in healthy humans: a systematic review and meta-analysis’, Journal of the European Academy of Dermatology and Venereology. doi: 10.1111/jdv.18123.

López-Otín, C. et al. (2023) ‘Hallmarks of aging: An expanding universe’, Cell. doi: 10.1016/j.cell.2022.11.001.

López de Figueroa, P. et al. (2023) ‘Lamin A/C Accumulation By Defective Autophagy Is Associated With Chondrocyte Senescence And Joint Damage’, Osteoarthritis and Cartilage, 31. doi: 10.1016/j.joca.2023.01.357.

Malbos, D. (2025) ‘Management of dyslipidemia’, Actualites Pharmaceutiques, 64(651). doi: 10.1016/j.actpha.2025.09.009.

Marta Sánchez, Elena González-Burgos, I. I. and M. P. G.-S. (2020) ‘Pharmacological Update Properties of Aloe Vera and its Major Active Constituents’, Molecules, 25(6). doi: doi: 10.3390/molecules25061324.

Mohite, S.-K. et al. (2021) A Review Article on: Aloe Vera : Extraction of Gel and Extraction of Aloin From Aloe Vera Gel by Ultrasonic Assisted Method, International Journal of Creative Research Thoughts.

Nussbaumerova, B. and Rosolova, H. (2023) ‘Obesity and Dyslipidemia’, Current Atherosclerosis Reports. doi: 10.1007/s11883-023-01167-2.

Rong, L. et al. (2023) ‘Advancements in the treatment of non-alcoholic fatty liver disease (NAFLD)’, Frontiers in Endocrinology. doi: 10.3389/fendo.2022.1087260.

Seliem, A. O. et al. (2024) ‘An Overview on Histology of the skin’, International Journal of Multiphysics, 18(3).

Vani, ade teti (2022) GEL ALOE VERA - Google Play Books. 1st edn. Edited by M. Silpi, D. hasbi Rahman, and B. Anggreno. Indramayu: Adab. Available at: https://play.google.com/books/reader?id=cmBZEAAAQBAJ&pg=GBS.PR2&lr=&printsec=frontcover (Accessed: 2 February 2022).

Wilkinson, H. N. and Hardman, M. J. (2020) ‘Wound healing: cellular mechanisms and pathological outcomes’, Open Biology, 10(9). doi: 10.1098/RSOB.200223.